301843 UE Practical Course in Molecular and Cellular Immunobiology (2023S)
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Registration/Deregistration
- Registration is open from Th 09.02.2023 08:00 to Tu 30.05.2023 09:00
- Deregistration possible until Tu 30.05.2023 09:00
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Lecturers
Classes (iCal) - next class is marked with N
Dates see german Version!Ort: BZB, Dr. Bohr-Gasse 9, 1030 Wien.Curricular requirements for enrollment to the course for all participants: Completion of Lecture 301364 (Research topics in Molecular Microbiology, Microbial Ecology and Immunobiology).;Course requirements and admission criteria:1. Attendance at the preliminary meeting (‘Vorbesprechung’);2. Master’s students in A 066 830 'Molecular Microbiology, Microbial Ecology and Immunobiology' will have preferential access;3. Additional requirements for students who have not completed their bachelor's degree in the bachelor's program Biologie at the University of Vienna in the areas of BMG or BMB: External students who got practical courses (UE) as requirement by the admission to the Master´s program have to complete them in advance (bring a copy of your admission letter and a current transcript to the preliminary meeting).4. Students from other disciplines must have completed an introductory course in immunology (e.g., Immunology and Cellular Microbiology A).
a. Aims: Students should learn immunological techniques, e.g. working with cell culture (sterile handling of cell lines, transfection of cells, isolation and differentiation of primary cells from bone marrow and spleen), antiviral assays, fluorescence microscopy, luciferase assays, FACS, purification of antibodies, immune precipitation, Western blot. Acquiring knowledge and experimental skills concerning the innate immune system, and functional assays on genetically modified mammalian cells.
b. contents: In this course part we will use CRISPR-Cas9 technology to make knock-outs of key genes of the innate immune system in human cells. The generated cells will be functionally tested using reporter assays.