Universität Wien FIND

301845 UE Practical Course in Molecular Microbiology (2020S)

10.00 ECTS (6.00 SWS), SPL 30 - Biologie
Continuous assessment of course work


Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).


max. 8 participants
Language: English


Classes (iCal) - next class is marked with N

First meeting: 4.3.2020, 19.00 Uhr SR 3/6.Stock-BZB, Dr.Bohrgasse 9,1030 Wien.
Exact term will be fixed at the first meeting(will be during the summerholidays)
requirement is the lecture Pathogene Bakterien in WS !
the course will be at the VetMed 1210 Wien.

Für externe Studierende: Zulassungsvoraussetzungen für diese Übungen sind prüfungsimmanente Lehrveranstaltungen (UE) der Auflagen zur Studienzulassung.
For external students: requirements for this practical course are the completion of compulsory courses with continuous assessment (UE) from the admission certificate.
Weitere Infos: http://molekularebiologie.univie.ac.at/

Wednesday 04.03. 19:00 - 20:00 BZB EDV-Raum CCR01, 6.Ebene 6.505, Dr.-Bohr-Gasse 9, 1030 Wien (Kickoff Class)


Aims, contents and method of the course

In this course the students have to classify and to identify unknown bacterial isolates which are subjected to a set of different methods nowadays applied in bacterial systematics. These methods include a) classical approaches such as Gram-staining, tests for Oxidase and Katalase, utilisation of carbon sources and production of acid from sugars; b) chemotaxonomic methods such as analysis of quinones, polar lipids, mycolic acids, cell wall diamino acids and polyamines; c) DNA-based methods including generation of genomic fingerprints after ERIC-, REP- and BOX-PCR, restriction analysis of amplified ribosomal DNA (ARDRA) and comparison of sizes of DNA amplicons obtained after amplification of the 16S-23S interspacers (ITS). Based on the results the bacterial isolates are classified in comparison with data from literature and allocated to genera or species.

Assessment and permitted materials

The evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam.

Minimum requirements and assessment criteria

Compulsory attendance;
- Active participation and results obtained (50%),
- Written report (30%),
- Theoretical knowledge including final exam (20%);
Each of the subgrades must have a positive evaluation.

Examination topics

Contents and theoretical background of the course units.

Reading list

Course manual

Association in the course directory


Last modified: We 30.11.2022 00:27