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301854 UE Practical Course in Structural Biology B (2019W)

Advanced Practical Course

5.00 ECTS (4.00 SWS), SPL 30 - Biologie
Continuous assessment of course work



max. 20 participants
Language: English



Place: Department of Structural and Computational Biology, Campus-Vienna-Biocenter 5, 1030 Vienna, room 1113

Time: 2 - 13 December 2019, all day long

Start: 2 December 2019, 10:00 am

No pre-meeting: Interested students come directly to the beginning of the practical

Knowledge in structural biology, NMR spectroscopy, X-ray crystallography, Practical Course in Structural Biology A - Basics (301853 UE). Previous attendance at the course Macromolecular Crystallography (301450 VO) and/or Advanced Macromolecular Crystallography (301449 VO) is strongly recommended.
For external students: Admission requirements for these exercises are courses with continous assessment (UE) that are required for admission to the study.


Aims, contents and method of the course

The lecture deals with the methodology and applications of NMR spectroscopy to relevant problems of molecular biology. The basic principles of the methodology are explained and demonstrated with practical applications to structural biology.
During the exercises students will perform the protein crystallisation and collection of x-ray diffraction data. In addition, they will solve the protein crystal structure by applying single-wavelength anomalous dispersion with the anomalous signal from sulfur atom (S-SAD). Finally, they will build, refine and evaluate the determined protein structure.

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, written report, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.

Minimum requirements and assessment criteria

Theoretical knowledge in basics of structural biology, spectroscopy, and diffraction theory, as well as active participation in conducting experiments and their careful evaluation.

Examination topics

Reading list

Association in the course directory

MMB II-3, MMB II-4, MMB W-2, MMB W-3, M-WZB, CHE II-5, PhD MB 1

Last modified: Mo 14.10.2019 09:08