Universität Wien FIND
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301901 VO+UE Introduction into 3D cryo electron microscopy (2019S)

for diploma, PhD and advanced students

3.00 ECTS (2.00 SWS), SPL 30 - Biologie
Continuous assessment of course work

Details

max. 25 participants
Language: English

Lecturers

Classes

jeweils Do. 17.00 - 18.30 Uhr
Beginn 4.4.2019
Raum 1.018/IMP, Viennabiocenter 1, 1030 Wien.
Abschlussprüfung im Juli
Weitere Infos: http://molekularebiologie.univie.ac.at/


Information

Aims, contents and method of the course

Mathematical Background
Fourier Theory for 3D EM
Correlation and Convolution
Information theory for 3D EM

Optics of the Transmission electron microscope
The electron source
The condenser system
The objective and image generation within an electron microscope
Electron detection

Sample preparation

Image processing
Single particle 2D analysis
Single particle 3D generation
Single particle refinement and validation
Tomographic processing

Modern concepts

Practicals/Tutorials:

Negative staining EM

Cryo EM

Tomographic acquisition

Single particle image processing

Tomographic image processing

Assessment and permitted materials

Compulsory attendance; the evaluation consists of different performances: active participation and results obtained, theoretical knowledge including final exam (the percentage of the subgrades will be announced by the course leader); each of the subgrades must have a positive evaluation.
Final exam / Testat in practical

Minimum requirements and assessment criteria

Grundverstaendis der Konzepte soll erworben werden

Examination topics

Reading list


Association in the course directory

PhD-MB 2, WZB, MMB W-2, MMEI III

Last modified: Mo 24.06.2019 16:29