320039 VO Separation and analysis methods for organic drug compounds (2012S)

In the first part, analytical methods of particular relevance for pharmacy laboratories are presented, the second part deals with instrumental separation techniques with a focus on methods for the separation of classical (small-molecule) drug compounds.
Aim: Acquisition of basic knowledge on the qualitative analysis of drug compounds with methods available in a pharmacy laboratory (proof of identity for drug compounds); knowledge about principles of function, applicability, and limitations of instrumental separation techniques (focus on chromatographic methods).
Contents: Determination of elemental composition, solubility, precipitation reactions, color reactions, identification of functional groups in drug compounds, physical properties, analysis of medicinal products (extraction of drug compounds, separation procedures); principles of chromatographic methods (separation mechanisms, classification of methods, theories of the chrom. process); individual chrom. methods: thin layer chromatography (TLC), column chrom. (CC), high performance liquid chrom. (HPLC), gas chrom. (GC); for each method: characteristic features, instrumentation, development techniques, detection methods, sample preparation, derivatisation; pharmaceutical applications; determination of separation performance, quantitative aspects (quantitation of elution curves and inner chromatograms, employment of internal and external standards); validation of chromatographic methods; reiated methods (SFC, CE, CEC).
Preconditions: successful completion of courses of the first study section; examinations on lectures from the second study section (3rd and 4th semester) can be done anyway (cf. Studienplan).
Mode of examination: written examination