320106 PR Arzneistoffanalytik (6 Kurse) (2005W)

The course is aimed at teaching practical experience in defining the elemental composition of drugs using chemical and chromatographic methods. The teaching programme contains elemental analysis of various drugs and the identification of functional groups. Unknown substances should be identified using a melting point apparatus. Additionally thin layer chromatography should be used to identify pharmaceutical drugs. Using the refraction index detector the students are expected to analyse the refraction index of a pure substance and to measure the concentration of a saccharose solution. The concentration of various vitamin solutions has to be determined by using spectrophotometric data. The students should learn how to use column chromatography to isolate a drug compound from a matrix. Identity and purity of the isolated compound are verified via IR-spectroscopy (interpretation by comparison with IR data given). Separation of purine derivatives via HPLC should demonstrate the many possibilties of HPLC compared to the method of column chromatography. In addition to that, NMR-spectra, IR and mass data should be used to identify the structure of unknown organic compounds.