320113 VO Cell culture models in Pharmaceutical Technology (2009S)

ECTS-points: 1.0
Aim:
to give detailed information about the principles und application of pharmaceutical cell culture models, to establish a basis for self-dependent cell culture work

Contents:
basic characteristics of the mammaliancell (structure, molecular organisation, physiology)

cell culture models in pharmaceutical analysis, suitable cell lines (Caco-2, HT29, ...- characteristics, special features, application)

guidelines for cultivation of cells (materials and methods, practical application, precautions), tissue-models (preparation of monolayers, differentiation, trans-well-systems, TEER-measurements, marker compounds)

application of pharmaceutical cell cultures (potentials, limits, comparison between in vitro and in vivo - systems), experimental setup (single cells, monolayers, trans-well-systems)

analytical techniques using cell cultures: proliferation studies (MTT, BrdU), viability tests (trypane blue, ethidium bromide), fluorescence-reader, flow-cytometry, confocal laser scanning microscopy

selected examples for the pharmaceutical application of Caco-2 cells (binding studies, competitive inhibition of cellular interactions, differences single cell/monolayer, transport studies)

Prerequisites: none
Examination: oral