Universität Wien FIND

321023 VO Methods of Separation and Analysis of Org. Drugs incl. Bioanalytical Methods - BA12 (2018W)

5.00 ECTS (3.00 SWS), SPL 32 - Pharmazie

Vorlesungszeiten:
01.10.2018 - 31.10.2018
Montag - Freitag 11.30 - 13.00 Uhr HS 6
07.11./08.11./09.11./14.11. 11.30 - 13.00 Uhr HS 6

Registration/Deregistration

Details

Language: German

Lecturers

Classes (iCal) - next class is marked with N

Monday 01.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Tuesday 02.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 03.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Thursday 04.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Friday 05.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Monday 08.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Tuesday 09.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 10.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Thursday 11.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Friday 12.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Monday 15.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Tuesday 16.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 17.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Thursday 18.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Friday 19.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Monday 22.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Tuesday 23.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 24.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Thursday 25.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Monday 29.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Tuesday 30.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 31.10. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 07.11. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Thursday 08.11. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Friday 09.11. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG
Wednesday 14.11. 11:30 - 13:00 UZA2 Hörsaal 6 (Raum 2Z227) 2.OG

Information

Aims, contents and method of the course

The first part of the lecture deals with pharmacy-lab relevant methods for the analysis of drug compounds (APIs) and with the pharmaceutically relevant separation methods, especially for small molecules. In the second part of the lecture, established methods for the analysis of (pharmaceutically relevant) biomolecules are treated, with a special focus on proteins and nucleic acids.

Aims of part 1: providing basic knowledge and understanding of qualitative analytics of drug compounds with methods that can be applied in a community pharmacy (identity check of APIs); knowledge about the underlying priciples, scope and limitations of separation techniques used in instrumetal abalytics (focus: chromatographic methods).

Aims of part 2: providing basic knowledge of analytical methods used in the bio sciences. These methods are essential both in the production and quality assurance of pharmaceutical products known as “biologicals” (proteins, glycoproteins, drugs produced with the aid of recombinant DNA,…) as well as in the diagnosis of diseases (genomics, DNA chips).

Contents of part 1: determination of the elemental composition, solubility, precipitation reactions, colour reactions, proof of functional groups in drug molecules, physicochemical properties (melting point, refraction index); chromatographic methods (underlying mechanism, classification, theoretical principles); different experiemntal thechniques: thin-layer chromatography (TLC), column chromatography (CC), high-performance liquid chromatography (HPLC), gas chromatography (GC); discussion of their characteristic features, necessary equipment, development techniques, detection options, sample preparation, derivatisation; examples of pharmaceutical applications; determination of the separation performance of a chromatographic system; quantitative aspects in chromatography, working with internal and external standards; validation of methods

Contents of part 2: I. Peptides and proteins: Basics, synthesis of peptides, purification of proteins, electrophoresis, methods of structure determination, quantification, chemical modification, radioactive labeling of proteins; proteomics; glycoproteins.
II. Nucleic acids: Basics, synthesis of oligonucleotides, purification of nucleic acids, analytical methods (hybridization, DNA sequencing, DNA chips).
III. Enzymatic analysis
IV. Biosensors

Methods used in the lecture: face-to-face presentation with PowerPoint projection and auxiliary visualisation tools (video, Flash animation)

Assessment and permitted materials

Written examination without any aids

Minimum requirements and assessment criteria

Duration of the exam: 60 min. A maximum of 24 points out of 12 questions (6 from each part) can be achieved, a minimum of 13 points (>50%) is required for a positive mark. Moreover, at least 6 points from each of the lecture‘s two parts must be achieved.

Examination topics

Subject of the exam is the entire material that is presented in the lecture. Sufficient knowledge of functional groups and common trivial names is expected. Moreover, basic knowledge about the structure of proteins and nucleic acids is required.

Reading list

PDF download from Moodle LMS

Association in the course directory

A207, A209, BA23

Last modified: Tu 18.09.2018 14:28