322053 VU Recombinant protein expression, purification and characterization by NMR - M14 (2024W)
Continuous assessment of course work
Labels
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 26.09.2024 12:00 to Mo 13.01.2025 12:00
- Deregistration possible until Mo 13.01.2025 12:00
Details
Language: English
Lecturers
Classes
20.01.2024 9:00 -15:00
21.01.2024 9:00 -15:00
22.01.2024 9:00 -15:00
23.01.2024 9:00 -15:00
24.01.2024 9:00 -15:00
27.01.2024 9:00 -15:00
28.01.2024 9:00 -15:00
Information
Aims, contents and method of the course
Assessment and permitted materials
The students will be assessed on their active participation during the course of the practicum as well as through a final report.
Minimum requirements and assessment criteria
In order to attend the course, the students needs to have basic laboratory experience as well as a superficial knowledge of protein biochemistry.
Examination topics
Content of the practical
Reading list
Recombinant protein expression in Escherichia coli: advances and challengesG. L. Rosano and E. A. CeccarelliFrontiers in Microbiology 2014 Vol. 5DOI: 10.3389/fmicb.2014.00172https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00172Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Association in the course directory
MA23
Last modified: Tu 12.11.2024 15:26
Through the course of this practicum, the students will gain an overview and hands on experience of the methods of recombinant protein expression. They will use state of the art techniques that are used in academia and industry with applications in biochemistry, structural biology, protein engineering, biotechnologies, pharmacy etc.