322087 VO 3R (Replacement-Reduction-Refinement)-Konzepte für den optimierten Einsatz von Tierversuchen - M14 (2025S)

Winfried Neuhaus:
Introduction into the 3Rs world and concepts (EU initiatives and regulations, topic animal experiment numbers, 3Rs centers, data bases, conferences, topics, ethics,..), Reproducibility crisis of biomedical science (in vitro/in vivo correlations, species differences), overview about the lecture

Marietta Zille:
Examples of in vitro alternatives as replacement strategies (e.g., skin corrosion and irritation, skin sensitization, eye irritation and corrosion, genotoxicity/mutagenicity, phototoxicity) and which in vivo tests they replace
In silico tools for structural alerts for drug discovery

Beate Rinner:
Improved preclinical Imaging and applications for refinement and reduction (cell tracking, contrast agents, ultrasound guided injection), human autologous tumor models and orthotopic mouse models.

Regina Grillari:
Good Cell Culture Practice GCCP1.0 and 2.0 and Good In Vitro Method Practice GIVIMP; ethical requirements for work with human cells and tissues, risk assessment when working with human cell cultures, quality control testings and standardization of in vitro cell culture work
Comparison of primary cells, in vitro immortalized cells, tumor cells, advantages and disadvantages of these different cell systems

Doris Willingseder:
Road from 2D to 3D; spheroids; hanging drops; perfusion and microfluidic technologies; organoids; organ-on-chips; 3D bioprinting; high content screening; advantages and disadvantages of the different systems

Roberto Plasenzotti:
Refinement I, Good animal experimentation practice, PREPARE concept, aspects of breeding: Biosafety/zoonoses, transgenic breeding, animal hygiene, best practice examples for 3R implementation

Birgit Reininger-Gutmann:
Refinement II, Animal experimentation law, ethics, best practise examples for 3R implementation