Universität Wien
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330068 SE Qualitative and quantitative bioanalytics related to chromatographic methods with main focus on HPLC (2021W)

Continuous assessment of course work

Lehrveranstaltung für Master- und Doktoratsstudium

Die VO Biostatistik für Fortgeschrittene sollte als Vorbereitung zu diesem SE bereits absolviert sein.

Anmeldung über Univis ist für eine Beurteilung unbedingt erforderlich.

Im Falle einer VERHINDERUNG an der Teilnahme ist eine ABMELDUNG innerhalb der Abmeldefrist UNBEDINGT ERFORDERLICH, da bei Abbruch und unentschuldigtem Fernbleiben eine negative Bewertung erfolgt.
Abmeldungen außerhalb dieser Frist können nur mit Nachweis eines triftigen Grundes (z.B. Erkrankung) erfolgen.


Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).


max. 15 participants
Language: German


Classes (iCal) - next class is marked with N

Monday 04.10. 15:30 - 16:30 Digital (Kickoff Class)


Aims, contents and method of the course

1. General Introduction to Chromatography (separating principles, used phases, parameters, methods, detectors)

2. Learning about different chromatographic methods
(thin layer-chromatography, liquid-chromatography, gas-chromatography)

3. Main focus HPLC (High-pressure-liquid-Chromatography)
-stationary phase / mobile phase
-separating column (principle, function)
-UV/VIS, fluorescence detection
-pump (isocratic, gradient)
-sampler (injector, autosampler)
-evaluation of data
-interpretation of data
-creating a separating-assay
-trouble shooting

4.practical demonstrations using devices

Assessment and permitted materials

Final part of the lecture will be a Powerpoint-presentation of each participant of 10 minutes with additional discussion.

Grading key is as follows:
40% presence (minimum 80%)
20% participation
40% final presentation

A postitive grade is only given, if all criteria are fulfilled.

Minimum requirements and assessment criteria

Students should become familiar with HPLC. They should understand the background and work on chromatographic problems themselves. Students should be able to operate on a HPLC device.

Examination topics

The lecture should be known as interactive exchange between lecturer and students. The immanent character of the lecture is to demand the students maximally. Illustrative material as well as video-clips support the learning effect.

Reading list

Wird nach den einzelnen Unterichtstagen als pfd-Datei zur Verfügung gestellt.

Association in the course directory

Last modified: Fr 12.05.2023 00:24