560060 UE Practical Course in fluorescence-/confocal microscopy including image processing workshop (2021S)
Vienna Biocenter PhD Program
Continuous assessment of course work
Labels
MIXED
Summary
Registration/Deregistration
Note: The time of your registration within the registration period has no effect on the allocation of places (no first come, first served).
- Registration is open from Th 11.02.2021 08:00 to Th 25.02.2021 18:00
- Deregistration possible until We 31.03.2021 18:00
Registration information is available for each group.
Groups
Group 1
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes (iCal) - next class is marked with N
-hybrid teaching-
Preliminary meeting (to be held as digital Collaborate meeting): ;https://eu.bbcollab.com/guest/119d87fc7fba4669a6b0f59062112d0f
Wed, March 17th, 10.30-11.00am:
Attendance mandatory!Registration via u:space for ONE of the two offered courses is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis)# of Attendees limited to 8.Details to program and course workflow in the preliminary meeting.Course Dates: May 18th-20th, 26th and 27th; June 1st, 2nd, 8th and 9th, 2021.
PHYSICAL Attendance on these days mandatory:
Course 1: May 26th+27th; June 8th
Course 2: June 1st, 2nd, and 8thLocation: VBC5, Dr.Bohrgasse 9, 1030 Vienna; level E1To participate in on-site courses and exams, you have to present a negative COVID-19 test result. Accepted tests and confirmations are:
Antigen test by a recognised test centre: Sample taken no more than 48 hours before the course unit/exam,
PCR test by a recognised test centre, including “Alles gurgelt” (everybody gargle): Sample taken no more than 72 hours before the course unit/exam,
PCR test for employees at the University of Vienna: Sample taken no more than 72 hours before the course unit/exam,
laboratory test for neutralising antibodies against COVID-19: valid for three months from the date of the test
Medical certificate of recovery from a COVID-19 infection within the past six months before the course unit/exam or
Official order ("Absonderungsbescheid") after the period of quarantaine within the past six months before the course unit/exam
Please have your confirmation/test result ready during your stay at the University. You may be asked to present it. The document remains with you.
If you cannot present a valid confirmation/test result, you are not allowed to participate in on-site courses/exams.
You can find recognised test centres under Österreich testet
Make use of the “Alles gurgelt” (everybody gargle) campaign.UPDATE NOV 19th:
For participation on-site to all physical presence units EVERYBODY has to provide a PCR test certificate NO LONGER than 48 hours old. THIS ALSO APPLIES FOR VACCINATED AND RECOVERED PERSONS!
- Tuesday 18.05. 10:30 - 12:30 Digital
- Wednesday 19.05. 10:00 - 12:00 Digital
- Thursday 20.05. 10:30 - 12:30 Digital
- Wednesday 26.05. 09:30 - 17:30 Hybride Lehre
- Thursday 27.05. 09:30 - 17:30 Hybride Lehre
- Tuesday 08.06. 10:00 - 15:00 Hybride Lehre
- Wednesday 09.06. 10:00 - 15:00 Hybride Lehre
Assessment and permitted materials
oral exam and continuous assessment
Group 2
In case non-German speaking students participate we, of course, will teach in English!
max. 8 participants
Language: German, English
LMS: Moodle
Lecturers
Classes
-hybrid teaching-
Preliminary meeting (to be held as digital Collaborate meeting):LINK: https://eu.bbcollab.com/guest/119d87fc7fba4669a6b0f59062112d0f
Wed, March 17th, 10.30-11.00am:
Attendance mandatory!Registration via u:space for ONE of the two offered courses is SUFFICIENT.
Simple registration does NOT necessarily lead to a fixed ticket for the course.
In case of over-registration the course head will select people dependent on their career advancement and the actual demand (e.g. for a running thesis)# of Attendees limited to 8.Details to program and course workflow in the preliminary meeting.Course Dates: May 18th-20th, 26th and 27th; June 1st, 2nd, 8th and 9th, 2021.
PHYSICAL Attendance on these days mandatory:
Course 1: May 26th+27th; June 8th
Course 2: June 1st, 2nd, and 8thLocation: VBC5, Dr.Bohrgasse 9, 1030 Vienna; level E1To participate in on-site courses and exams, you have to present a negative COVID-19 test result. Accepted tests and confirmations are:
Antigen test by a recognised test centre: Sample taken no more than 48 hours before the course unit/exam,
PCR test by a recognised test centre, including “Alles gurgelt” (everybody gargle): Sample taken no more than 72 hours before the course unit/exam,
PCR test for employees at the University of Vienna: Sample taken no more than 72 hours before the course unit/exam,
laboratory test for neutralising antibodies against COVID-19: valid for three months from the date of the test
Medical certificate of recovery from a COVID-19 infection within the past six months before the course unit/exam or
Official order ("Absonderungsbescheid") after the period of quarantaine within the past six months before the course unit/exam
Please have your confirmation/test result ready during your stay at the University. You may be asked to present it. The document remains with you.
If you cannot present a valid confirmation/test result, you are not allowed to participate in on-site courses/exams.
You can find recognised test centres under Österreich testet
Make use of the “Alles gurgelt” (everybody gargle) campaign.
Assessment and permitted materials
oral exam and continuous assessmen
Information
Aims, contents and method of the course
Minimum requirements and assessment criteria
The aim of the course is to give theoretical and practical support to students, who are or will in the near future be involved with light microscopical experiments within their Master/PhD/post-doc studies. The small groups (2 people) will allow to advice students somehow personally for their planned experimental approaches.
Examination topics
microscopy sample preparation
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
widefield fluorescence microscopy (incl. Köhler and contrast enhancement)
measuring point spread function and chromatic aberration
confocal microscopy
image processing using "huygens" and ImageJ software
Reading list
J.Pawley, Handbook of Biological Confocal Microscopy, Springer, 2006
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Yuste, Imaging – A Laboratory manual; CSHL-Press, 2010
D.B.Murphy, Fundamentals of Light Microscopy and Electronic Imaging, Wiley, 2001 / 2009
E.M.Goldys, Flurescence Applications in Biotechnology and the Life Sciences, Wiley, 2009
Kevin F. Sullivan, Fluorescent Proteins (Methods in Cell Biology) , Academic Press, 2008
Association in the course directory
PhD-MB 2, MMEI III, MMB W-2, WZB
Last modified: Fr 12.05.2023 00:27
technical description of equipment
Wet lab experience sample preparation
Quality criteria for dealingwith aberrations correction
practical microscopy of selected samples by fluorescence- and confocal microscopy (multichannel detection, serial optical sections through samples, image projections, generation of time series)image analysis and deconvolution of selected images