801484 UE Chemical Laboratory Course for Biologists III (2003W)

In turn tutorials to the theoretical background and/or the experimental
procedures, followed by experiments singly, or in pairs, or in groups.
Introduction to the lab bench equipment. Law of mass action. Acids and
bases. Volumetric determinations. Buffer systems. Red-Ox reactions. Gas
volumetry. Ion selective electrodes.
Qualitative organic analysis: hetero elements, functional grops,
preparation of derivatives, determination of boiling points, refractory
indices and melting points.
Spectroscopy : 1. Infrared spectroscopy; 2. Nuclear magnetic resonance
spectroscopy.
Preparative organic chemistry: Synthesis of nitromethane.
Chromatography: 1. Gas chromatography
Distillation.
Chemistry of natural products: Carbohydrates. Amino acids, peptides and
proteins.
Chromatography: 2. Thin layer chromatography. 3. Ion exchange.
Chemistry of natural products: Isolation of leaf pigments.
Chromatography: 4. Column chromatography.
Spectroscopy : 3. Ultraviolet and visible light spectroscopy; 4. Mass
spectroscopy.
Chemistry of natural products: Isolation of fats and of flavoring
components from nutmeg.