877574 VO Angewandte Pharmakogenetik (2003W)
Angewandte Pharmakogenetik
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This course is intended to provide insights into the methodology of pharmacogenetics.
The pharmacokinetics of many drugs is affected by activity of specific cytochrom-P450-enzymes. Genotyping of test subjects (the course participants) will be performed by analysing genomic DNA. The polymorphism of cytochrom-P450 2D6 concerning a particular mutation will be determined. The therapeutic relevance of Cytochrome P450 polymorphism will be discussed.
The pharmacokinetics of many drugs is affected by activity of specific cytochrom-P450-enzymes. Genotyping of test subjects (the course participants) will be performed by analysing genomic DNA. The polymorphism of cytochrom-P450 2D6 concerning a particular mutation will be determined. The therapeutic relevance of Cytochrome P450 polymorphism will be discussed.
Examination topics
Reading list
Association in the course directory
N2270
Last modified: Fr 31.08.2018 09:01
" Isolation of leucocytes from blood samples and DNA extraction
" Primer design for amplification of the region of interest of the cytochrome P450 2D6 gene
" Amplification of the region of interest by polymerase chain reaction
" Purification of the amplified DNA fragment and DNA digestion with restriction endonucleases
" DNA separation by agarose gel electrophoresis and gel documentation
ECTS-points: 1.1