877729 SE+UE Fluorescence in situ hybridization (FISH) (2004S)
Identification of uncultivated microorganisms by fluorescence in situ hybridisation (FISH)
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Blockveranstaltung: 01.07 - 09.07.04 im Labor 2301. Max. 10 Teilnehmer. Vorbesprechung: Di 02.03.2004 um 12 Uhr im Hörsaal II
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Language: German
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Last modified: Fr 31.08.2018 09:01
This laboratory course will teach attendants how to specifically detect and identify prokaryotes in environmental and medical samples using the full cycle rRNA approach and FISH with rRNA-targeted oligonucleotide probes. The experiments performed will provide in-depth insights into parameters relevant for nucleic acid hybridization techniques and will expose students to epifluorescence- and confocal laser scanning microscopy. In addition, attendants will learn how to apply digital image analysis to extract quantitative data from FISH experiments.